Department Website
Head
Staff
- prof. dr hab. Jolanta Zakrzewska-Czerwińska
- dr hab. Marcin Szafran
- dr inż. Marcin Wolański
- dr Bernhard Kepplinger
- dr Martyna Gongerowska-Jac
- dr Agnieszka Strzałka
- dr inż. Joanna Hołówka
- dr Monika Pióro
- dr Tomasz Łebkowski
PhD Students
(rooms: 2.24, 2.25, 3.18)
- mgr Katarzyna Pawlikiewicz
- mgr inż. Marta Derkacz
- mgr Patrycja Wadach
- mgr Jakub Mikołajczyk
- mgr Anna Musiolik
- mgr Kamil Sobarnia
- mgr Kinga Klich
- mgr Elżbieta Kryska
- mgr Dominik Bania
RESEARCH OVERVIEW
Investigation of chromosome architecture and key stages of the cell cycle (chromosome replication and segregation, cell division) in Gram-positive bacteria belonging to actinomycetes, Streptomyces, and Mycobacterium.
LAB EQUIPMENT
- Real Time PCR – StepOnePlus (Applied Biosystem), Eco Real-Time PCR System (Illumina);
- UV/Vis spectrophotometers – Nano-drop ND-1000, Pharmacia Biotech;
- Centrifuges: AVANTI® J-26 XPI (Beckman), Allegra X-22R (Beckman), 5415R (Eppendorf), Micro FC5515R (Ohaus);
- Incubators – Excella E-24R (New Brunswick Scientific), Infors HT Multitron, Binder CO2 incubator (Binder);
- Real time microbiology reader – Bioscreen C;
- Inverted fluorescence microscopes – Delta-Vision Elite with microfluidic CellASIC system, ONIX;
- Fluorescence microscope – Leica DM6 B;
- qPCR cabinet – Aura PCR (Bioair / Euroclone);
- Laminar flow hoods – BIO60 (Faster), Mars Safety Class 2 (ScanLaf);
- Low temperature freezers – C340 (New Brunswick Scientific), Igloo C820 (Telstar), MDF-DU502VH-PE (PHCbi);
- Drying cabinets and other microbiology incubators – Binder;
- Autoclaves –Varioklav 75S, Tutnauer 2840EL-D;
- Capillary electrophoresis – QIAxcel (Qiagen);
- Multimode plate reader – Infinite M Plex (Tecan);
- FPLC protein Purification system – Akta Start (GE / Cytiva);
- Gel visualization systems – ChemiDoc XRS+ (Bio-Rad), Smart Imaging (Vilber);
- Water purification system – Simplicity UV (Millipore)
Research Projects:
Ongoing
2024-2028: „Exploring molecular mechanisms controlling ParB-dependent chromosome rearrangements in Streptomyces.” (NCN, OPUS 25; nr 2023/49/NZ1/00781).
Principal Investigator – M. Szafran
2024-2028: “The interplay between nucleoid associated proteins and transcription factors at regulation of gene expression in antibiotic producing bacteria.” (NCN, OPUS 25, nr 2023/49/B/NZ2/00356)
Principal Investigator – D. Jakimowicz
2023-2027: “Antibiotic discovery by induction of inactive antibiotic gene clusters.” (NCN, OPUS 24; 2022/47/B/NZ1/00556)
Principal Investigator – B. Kepplinger
2022-2025: ” Novel two-component system in Streptomyces and its role in growth regulation and mediating interspecies competition.” (NCN, SONATA 17; nr 2021/43/D/NZ2/00284)
Principal Investigator – M. Gongerowska-Jac
2021-2025: “ Chromosome architecture and translocation in asymmetrically growing and dividing Mycobacterium.” (NCN, OPUS 19; nr 2020/37/B/NZ1/0055)
Principal Investigator – J. Zakrzewska-Czerwińska
2021-2024: “ How do antibiotic-producing bacteria cells grow and branch?” (NCN, SONATA 16; nr 2020/39/D/NZ1/00303)
Principal Investigator – B. Kepplinger
Former (recent 5 years)
2019-2022: „Spatial organisation of Streptomyces chromosome during sporulation – impact on chromosome segregation, cell division, spores viability and germination.” (NCN, OPUS 16).
Principal Investigator – D. Jakimowicz
2019-2022 „Two lysine posttranslational modifications, acetylation and pupylation and their role in the regulation of Streptomyces chromosome topology.” (NCN, SONATA 14).
Principal Investigator – M. Szafran
2019-2022: „Bdellovibrio bacteriovorus, a ‘living antibiotic’ – life cycle dynamics during predation on different pathogens.” (NCN, OPUS 15; no. 2018/29/B/NZ6/00539.” (NCN, OPUS 15).
Principal Investigator – J. Zakrzewska-Czerwińska
2018-2022: „Mechanism of mycobacterial chromosome segregation – the role of proteins interacting with ParA.”(NCN, OPUS 14; nr. 2017/27/B/NZ1/00823).
Kierownik projektu – D. Jakimowicz
2018-2021: “mIHF, a unique mycobacterial nucleoid associated protein and its role in chromosome organization and gene expression.” (NCN, SONATINA 2; nr. 2018/28/C/NZ1/00128).
Principal Investigator – J. Hołówka
2018-2021: „Interaction between proteins HupA and HupS during Streptomyces growth and stress respon.” (NCN, SONATINA 2; nr. 2018/28/C/NZ1/00241).
Principal Investigator – A. Strzałka
2019-2020: „Global analysis of AdpA-dependent gene expression in Streptomyces under fluctuating oxygen level.” (NCN, Miniatura 3).
Principal Investigator – M. Wolański
2018-2019: „Biological role of the DnaA protein phosphorylation in Streptomyces coelicolor.” (NCN, Preludium 13).
Principal Investigator – T. Łebkowski
2017-2020: „Lsr2 protein as a global chromosomal organizer and transcription factor in Mycobacterium – an interplay of Lsr2 with SMC complex and HupB.” (NCN, Opus 13).
Principal Investigator – J. Zakrzewska-Czerwińska
2017-2020: „The architecture of bacterial chromosome – the cooperation of topoisomerase I and segregation protein ParB in Streptomyces.” (NCN, Harmonia 8).
Principal Investigator – M. Szafran
2017-2019: „Genetic tool for identification proteins regulating supercoiling- sensitive promoter of gene encoding topoisomerase I from Streptomyces coelicolor.”(NCN, Preludium 12).
Principal Investigator – M. Gongerowska-Jac