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Department of Molecular Microbiology

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Department Website

Head

Staff

PhD Students

(phone: 71-375-2393, -2640, rooms: 3.17, 3.18)


RESEARCH OVERVIEW

Investigation of chromosome architecture and key stages of cell cycle (chromosome replication and segregation, cell division) in Gram-positive bacteria belonging to actinomycetes, Streptomyces and Mycobacterium, and in Gram-negative predatory bacterium Bdellovibrio bacteriovorus.

LAB EQUIPMENT

  • Real Time PCR – StepOnePlus (Applied Biosystem), Eco Real-Time PCR System (Illumina);
  • UV/Vis spectrophotometers – Nano-drop ND-1000, Pharmacia Biotech;
  • Centrifuges: AVANTI® J-26 XPI (Beckman), Allegra X-22R (Beckman), 5415R (Eppendorf), Micro FC5515R (Ohaus);
  • Incubators – Excella E-24R (New Brunswick Scientific), Infors HT Multitron, Binder CO2 incubator (Binder);
  • Real time microbiology reader – Bioscreen C;
  • Inverted fluorescence microscopes – Delta-Vision Elite with microfluidic CellASIC system, ONIX;
  • Fluorescence microscope – Leica DM6 B;
  • qPCR cabinet – Aura PCR (Bioair / Euroclone);
  • Laminar flow hoods – BIO60 (Faster), Mars Safety Class 2 (ScanLaf);
  • Low temperature freezers – C340 (New Brunswick Scientific), Igloo C820 (Telstar), MDF-DU502VH-PE (PHCbi);
  • Drying cabinets and other microbiology incubators – Binder;
  • Autoclaves –Varioklav 75S, Tutnauer 2840EL-D;
  • Capillary electrophoresis – QIAxcel (Qiagen);
  • Multimode plate reader – Infinite M Plex (Tecan);
  • FPLC protein Purification system – Akta Start (GE / Cytiva);
  • Gel visualization systems – ChemiDoc XRS+ (Bio-Rad), Smart Imaging (Vilber);
  • Water purification system – Simplicity UV (Millipore)

 Research Projects:

Ongoing

  • 2021-2025: „Chromosome architecture and translocation in asymmetrically growing and dividing Mycobacterium” (NCN, OPUS 19; no. 2020/37/B/NZ1/0055).
    Principal Investigator – J. Zakrzewska-Czerwińska

  • 2019-2022: „Spatial organisation of Streptomyces chromosome during sporulation – impact on chromosome segregation, cell division, spores viability and germination.” (NCN, OPUS 16, no. 2018/31/B/NZ1/00614).
    Principal Investigator – D. Jakimowicz

  • 2019-2022 „Two lysine posttranslational modifications, acetylation and pupylation and their role in regulation of Streptomyces chromosome topology” (NCN, SONATA 14, no. 2018/31/D/NZ1/00287).
    Principal Investigator – M. Szafran

  • 2019-2022: „Bdellovibrio bacteriovorus, a ‘living antibiotic’ – life cycle dynamics during predation on different pathogens” (NCN, OPUS 15; no. 2018/29/B/NZ6/00539).
    Principal Investigator – J. Zakrzewska-Czerwińska

  • 2018-2022: „Mechanism of mycobacterial chromosome segregation – the role of proteins interacting with ParA” (NCN, OPUS 14; no. 2017/27/B/NZ1/00823).
    Principal Investigator – D. Jakimowicz

  • 2018-2021: “mIHF, a unique mycobacterial nucleoid associated protein and its role in chromosome organization and gene expression” (NCN, SONATINA 2; no. 2018/28/C/NZ1/00128).
    Principal Investigator – J. Hołówka

  • 2018-2021: „Role of the interaction between HupA and HupS proteins during Streptomyces growth and stress response” (NCN, SONATINA 2; no. 2018/28/C/NZ1/00241).
    Principal Investigator – A. Strzałka


Former (recent 5 years)

NCN grants:

  • 2019-2020: „Global analysis of AdpA-dependent gene expression in Streptomyces under fluctuating oxygen level” (NCN, Miniatura 3).
    Principal Investigator – M. Wolański


  • 2018-2019: „Biological role of the DnaA protein phosphorylation in Streptomyces coelicolor” (NCN, Preludium 13).
    Principal Investigator – T. Łebkowski


  • 2017-2020: „Lsr2 protein as a global chromosomal organizer and transcription factor in Mycobacterium – an interplay of Lsr2 with SMC complex and HupB” (NCN, Opus 13).
    Principal Investigator – J. Zakrzewska-Czerwińska


  • 2017-2020: „The architecture of bacterial chromosome – the cooperation of topoisomerase I and segregation protein ParB in Streptomyces” (NCN, Harmonia 8).
    Principal Investigator – M. Szafran


  • 2017-2019: „Genetic tool for identification proteins regulating supercoiling- sensitive promoter of gene encoding topoisomerase I from Streptomyces coelicolor.” (NCN, Preludium 12).
    Principal Investigator – M. Gongerowska-Jac


  • 2017-2018: „Single-cell dynamics of the chromosome replication in predatory bacterium, Bdellovibrio bacteriovorus” (NCN, Preludium 12).
    Principal Investigator – Ł. Makowski


  • 2015-2018: „Chromosome topology as a global regulator of gene transcription – interplay between topoisomerases and nucleoid binding proteins in bacteria adaptation to stress conditions” (NCN, Opus 8).
    Principal Investigator – D. Jakimowicz


  • 2015-2018: „Cell cycle specific fine-tuning of bacterial chromosome segregation – dissection of ParA interactions in vitro and in vivo” (NCN, Harmonia 6).
    Principal Investigator – D. Jakimowicz


  • 2012-2018: „DYNAMICS OF CHROMOSOME ARCHITECTURE AND REPLICATION DURING DIFFERENT BACTERIAL GROWTH PHASES – comparison of two related actinomycetales: pathogenic Mycobacterium and non-pathogenic mycelial Streptomyces” (NCN, Maestro 2).
    Principal Investigator – J. Zakrzewska-Czerwińska


NCBiR grants:

  • 2015-2018: „Next Generation Immunosuppressants: Brasilicardin synthesized by Nocardia spp” (ERA-IB).
    Principal Investigator – J. Zakrzewska-Czerwińska


Publications