Department Head

• prof. dr hab. Mariusz Olczak

Staff Members

• dr Justyna Ciuraszkiewicz
• dr Dorota Maszczak-Seneczko
• dr Maciej Wiktor
• dr Agata Szalewicz
• dr inż. Bożena Szulc

PhD Students

• mgr Wojciech Wiertelak
• mgr Edyta Skurska

   Laboratory of our Department:

phone +48 71375 2394, room 3.39

Research Topics

• Analysis of structure and function of glycoprotein sugar moiety
• Characterization of plant purple acid phosphatases
• Characterization of plant phosphatases/phosphodiesterases (PPD)
• Analysis of human serprocidins
• Analysis of mammalian nucleotide-sugar transporters
• Identification and characterization of bird and reptile transferrins

Research Projects

• Functional analysis of SLC35A transporter family – substrate specificity and ability to interact with other proteins in the Golgi membrane;   (2015-2018).
  Head: prof. dr hab. Mariusz Olczak
• Characteristics of nucleotide sugar transporters SLC35A4 and SLC35A5;  (2015-2018) .
  Head: mgr Paulina Sosicka

Selected Publications

1. Szulc B, Sosicka P, Maszczak-Seneczko D, Skurska E, Shauchuk A, Olczak T, Freeze HH, Olczak M. Biosynthesis of GlcNAc-rich N- and O-glycans in the Golgi apparatus does not require the nucleotide sugar transporter SLC35A3. J Biol Chem. 2020 Sep 16:jbc.RA119.012362. doi: 10.1074/jbc.RA119.012362.
2. Wiertelak W, Sosicka P, Olczak M, Maszczak-Seneczko D. Analysis of homologous and heterologous interactions between UDP-galactose transporter and beta-1,4-galactosyltransferase 1 using NanoBiT. Anal Biochem. 2020 Mar 15;593:113599. doi: 10.1016/j.ab.2020.113599.
3. Shauchuk A, Szulc B, Maszczak-Seneczko D, Wiertelak W, Skurska E, Olczak M. N-glycosylation of the human β1,4-galactosyltransferase 4 is crucial for its activity and Golgi localization. Glycoconj J. 2020 Oct;37(5):577-588. doi: 10.1007/s10719-020-09941-z.
4. Olczak T, Sosicka P, Olczak M (2015) HmuY is an important virulence factor for Porphyromonas gingivalisgrowth in the heme-limited host environment and infection of macrophages. Biochem Biophys Res Commun, 2015, 467, 748-753.
5. Maszczak-Seneczko D, Sosicka P, Kaczmarek B, Majkowski M, Luzarowski M, Olczak T Olczak M (2015) UDP-galactose (SLC35A2) and UDP-N-acetylglucosamine (SLC35A3) transporters form glycosylation-related complexes with mannoside acetylglucosaminyltransferases (Mgats). J Biol Chem 290:15475-15486
6. Dörre K, Olczak M, Wada Y, Sosicka P, Grüneberg M, Reunert J, Kurlemann G, Fiedler B, Biskup S, Hörtnagel K, Rust S, Marquardt T (2015) A new case of UDP-galactose transporter deficiency (SLC35A2-CDG): molecular basis, clinical phenotype, and therapeutic approach. J Inherit Metab Dis 38:931-940
7. Benedyk M, Byrne DP, Glowczyk I, Potempa J, Olczak M, Olczak T, Smalley JW (2015) Pyocyanin, a contributory factor in haem acquisition and virulence enhancement of Porphyromonas gingivalisin the lung. PloS One 10(2):e0118319
8. Śmiga M, Bielecki M, Olczak M, Smalley JW, Olczak T (2015) Anti-HmuY antibodies specifically recognize Porphyromonas gingivalisHmuY protein but not homologous proteins in other periodontopathogens. PloS One 10(2):e0117508
9. Sosicka P, Jakimowicz P, Olczak T, Olczak M (2014) Short N-terminal region of UDP-galactose transporter (SLC35A2) is crucial for galactosylation of N-glycans. Biochem Biophys Res Commun 454:486-492
10. Ciuraszkiewicz J, Śmiga M, Mackiewicz P, Gmiterek A, Bielecki M, Olczak M, Olczak T (2014) Fur homolog regulates Porphyromonas gingivalisvirulence under low-iron/heme conditions through a complex regulatory network. Mol Oral Microbiol 29:333-353
11. Antonyuk SV, Olczak M, Olczak T, Ciuraszkiewicz J, Strange RW (2014) The structure of a purple acid phosphatase involved in plant growth and pathogen defence exhibits a novel immunoglobulin-like fold. IUCrJ 1:101-109
12. Gmiterek A, Wójtowicz H, Mackiewicz P, Radwan-Oczko M, Kantorowicz M, Chomyszyn-Gajewska M, Frąszczak M, Bielecki M, Olczak M, Olczak T (2013) The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis. PLoS One 8(7):e67719
13. Byrne DP, Potempa J, Olczak T, Smalley JW (2013) Evidence of mutualism between two periodontal pathogens: Co-operative haem acquisition by the HmuY haemophore of Porphyromonas gingivalisand the cysteine protease interpain A (InpA) of Prevotella intermedia. Mol Oral Microbiol 28:219-229
14. Maszczak-Seneczko D, Sosicka P, Olczak T, Jakimowicz P, Majkowski M, Olczak M (2013) UDP-N-acetylglucosamine transporter (SLC35A3) regulates biosynthesis of highly branched N-glycans and keratan sulfate. J Biol Chem 288:21850-21860
15. Olczak M, Maszczak-Seneczko D, Sosicka P, Jakimowicz P, Olczak T (2013) UDP-Gal/UDP-GlcNAc chimeric transporter complements mutation defect in mammalian cells deficient in UDP-Gal transporter. Biochem Biophys Res Commun 434:473-478
16. Wójtowicz H, Bielecki M, Wojaczyński J, Olczak M, Smalley JW, Olczak T (2013) The Porphyromonas gingivalisHmuY haemophore binds gallium(iii), zinc(ii), cobalt(iii), manganese(iii), nickel(ii), and copper(ii) protoporphyrin IX but in a manner different to iron(iii) protoporphyrin IX. Metallomics 5:343-351
17. Maszczak-Seneczko D, Sosicka P, Majkowski M, Olczak T, Olczak M (2012) UDP-N-acetylglucosamine transporter and UDP-galactose transporter form heterologous complexes in the Golgi membrane. FEBS Lett 586:4082-4087
18. Trindade SC, Olczak T, Gomes-Filho IS, Moura-Costa LF, Vale VL, Galdino-Neto M, Alves H, Carvalho-Filho PC, Sampaio GP, Xavier MT, Sarmento VA, Meyer R (2012) Porphyromonas gingivalisantigens participate differently in the proliferation and cell death of human PBMC. Arch Oral Biol 57:314-320
19. Wojaczyński J, Wójtowicz H, Bielecki M, Olczak M, Smalley JW, Latos-Grażyński L, Olczak T (2011) Iron(III) mesoporphyrin IX and iron(III) deuteroporphyrin IX bind to the Porphyromonas gingivalisHmuY hemophore. Biochem Biophys Res Commun 411:299-304
20. Smalley JW, Byrne DP, Birss AJ, Wojtowicz H, Sroka A, Potempa J, Olczak T (2011) HmuY haemophore and gingipain proteases constitute a unique syntrophic system of haem acquisition by Porphyromonas gingivalis. PLoS One 6(2):e17182
21. Olczak M, Ciuraszkiewicz J, Wójtowicz H, Maszczak D, Olczak T (2009) Diphosphonucleotide phosphatase/phosphodiesterase (PPD1) from yellow lupin (Lupinus luteus) contains an iron-manganese center. FEBS Lett 583:3280-3284
22. Wójtowicz H, Guevara T, Tallant C, Olczak M, Sroka A, Potempa J, Solà M, Olczak T, Gomis-Rüth FX (2009) Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis. PLoS Pathogens 5(5):e1000419